Journal: ACS chemical neuroscience
Article Title: Altered Secretion, Constitution, and Functional Properties of the Gastrointestinal Mucus in a Rat Model of Sporadic Alzheimer's Disease.
doi: 10.1021/acschemneuro.3c00223
Figure Lengend Snippet: Figure 3. Biochemical analysis of the GI mucus obtained from the rat model of sporadic AD induced by intracerebroventricular streptozotocin (STZ-icv) and the controls. (A) UV−vis spectra of mucus from the control and the STZ-icv animals (left) and the area under the curve reflecting dilution (right). (B) Absorbance of samples at 230, 260, and 280 nm, reflecting the concentration of salts, nucleic acids, and proteins. (C) Relationship between sample absorbance (log-transformed y-axis) and wavelength depicted for the demonstration of the relationship between the 400 nm peak and the UV region. (D) Model-derived estimates from the linear model reflecting the concentration of protein in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (E) Model-derived estimates from the linear model reflecting the signal intensity of AB; reflecting glycoprotein content) in the mucus of the STZ- icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (F) Mucin 2 (MUC2) dot blot, model-derived estimates from the linear model reflecting the concentration of MUC2 in the mucus of the STZ-icv and the controls (left) and the contrast illustrating the effect size (right). Mean estimates are accompanied by 95% confidence intervals. (G) Model- derived estimates from the linear model reflecting peak mastPASTA-obtained force (inversely correlated with lubrication capacity) in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (H) Model-derived estimates from the linear model reflecting the oxidation−reduction potential (ORP) in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (I) Model- derived estimates from the linear model reflecting the NRP-integrated density in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (J) Model-derived estimates from the linear model reflecting the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-derived reductive capacity (in equivalents of dithiothreitol [mM]) in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (J) Model-derived estimates from the linear model reflecting lipid peroxidation (estimated using the thiobarbituric acid reactive substances (TBARS) assay) in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals.
Article Snippet: Blocked membranes were incubated with the rabbit anti-MUC2 primary antibody (Boster Biological Technology, USA) diluted in the blocking buffer 500-fold for 24 h at 4 °C.
Techniques: Control, Concentration Assay, Transformation Assay, Derivative Assay, Dot Blot, TBARS Assay
Santa Cruz Biotechnology is a verified supplier 
![Figure 3. Biochemical analysis of the GI mucus obtained from the rat model of sporadic AD induced by intracerebroventricular streptozotocin (STZ-icv) and the controls. (A) UV−vis spectra of mucus from the control and the STZ-icv animals (left) and the area under the curve reflecting dilution (right). (B) Absorbance of samples at 230, 260, and 280 nm, reflecting the concentration of salts, nucleic acids, and proteins. (C) Relationship between sample absorbance (log-transformed y-axis) and wavelength depicted for the demonstration of the relationship between the 400 nm peak and the UV region. (D) Model-derived estimates from the linear model reflecting the concentration of protein in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (E) Model-derived estimates from the linear model reflecting the signal intensity of AB; reflecting glycoprotein content) in the mucus of the STZ- icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (F) <t>Mucin</t> <t>2</t> <t>(MUC2)</t> dot blot, model-derived estimates from the linear model reflecting the concentration of MUC2 in the mucus of the STZ-icv and the controls (left) and the contrast illustrating the effect size (right). Mean estimates are accompanied by 95% confidence intervals. (G) Model- derived estimates from the linear model reflecting peak mastPASTA-obtained force (inversely correlated with lubrication capacity) in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (H) Model-derived estimates from the linear model reflecting the oxidation−reduction potential (ORP) in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (I) Model- derived estimates from the linear model reflecting the NRP-integrated density in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (J) Model-derived estimates from the linear model reflecting the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-derived reductive capacity (in equivalents of dithiothreitol [mM]) in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals. (J) Model-derived estimates from the linear model reflecting lipid peroxidation (estimated using the thiobarbituric acid reactive substances (TBARS) assay) in the mucus of the STZ-icv and the controls (upper) and the contrast illustrating the effect size (lower). Mean estimates are accompanied by 95% confidence intervals.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_7640/pm37477640/pm37477640__page6_image1.jpg)